Table 3.
Current Ratios for “8oxoG” and “8OHdG” Peaks by CEEC
Peak ID and Sample | Detection Potential (mV) | Peak Height (pA) | Current Ratio (450 / 650) |
---|---|---|---|
8oxoG | |||
Basal dialysate | 450 | 2.1 ± 0.1 | |
0.51 ± 0.01 | |||
650 | 4.3 ± 0.1 | ||
20 nM standard | 450 | 7.0 ± 0.1 | |
0.48 ± 0.02 | |||
650 | 13.8 ± 0.3 | ||
8OHdG | |||
Basal dialysate | 450 | 0 | |
0 | |||
650 | 2.2 ± 0.4 | ||
20 nM standard | 450 | 3.6 ± 0.0 | |
0.38 ± 0.01 | |||
650 | 9.6 ± 0.3 |
Conditions: Sample or standard in Ringer’s injected for 60 s followed by 15 s injection of 0.1 M NaOH; Tris, borate BGE with 0.5 mM TTAB at pH 8.7, 50 µm i.d. capillary × 60 cm, separation and injection at −10 kV; detection potential vs. Ag/AgCl.