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. Author manuscript; available in PMC: 2009 Jan 1.
Published in final edited form as: Eur J Neurosci. 2008;27:2691–2700. doi: 10.1111/j.1460-9568.2008.06224.x

Fig. 1. Characterization of antibody AN519 (anti-Pdc-Ser73).

Fig. 1

A. Time course of purified bovine Pdc phosphorylation by PKA, analyzed by autoradiography and Western Blotting. B. Specificity of AN519 for phosphorylated bovine Pdc. Increasing amounts of phosphorylated (Pdc-pSer73) or non-phosphorylated (Pdc) bovine phosducin were subjected to Western blot analysis with AN519. C. Phosphorylation of Pdc in mouse retinal homogenate. Mouse retinal extract (40 μg) was incubated with ATP/Mg, okadaic acid, IBMX with (lanes 1, 3, 5) or without cAMP (lanes 2, 4) followed by SDS/PAGE. The proteins were visualized either by Coomassie brilliant blue (CBB) staining or Western blot analysis with AN519 or anti-Pdc-pan. See Materials and methods for details.