Table 1. List of 22 Target genes up-regulated following UPF1 silencing in HCT116 and their mutational status.

Gene Symbol	Ensembl Human Gene	Fold Change	Repeat	Repeat Position in cDNA	Type of Mutation in HCT116
ARV1	ENSG00000148926	2.08	9A	539	hmz
CDC23	ENSG00000094880	1.99	7A	411	wt
CSPG2	ENSG00000038427	2.17	7A	7874	wt
EFHC1	ENSG00000096093	1.94	7A	1316	htz
MBD4*	ENSG00000129071	1.89	10A	930	htz
MSH3*	ENSG00000113318	1.6	8A	1394	hmz
NR3C1	ENSG00000113580	1.87	7A	1974	wt
ORC6L	ENSG00000091651	2.24	7A	561	wt
PIGB	ENSG00000069943	1.85	8T	1086	wt
PSD3	ENSG00000156011	1.6	8A, 8A	737, 2333	wt
PSEN1	ENSG00000080815	2.02	7T	774	wt
REV3L	ENSG00000009413	1.91	8A	1481	wt
RIF1	ENSG00000080345	1.75	7T, 8A	1538, 4637	wt
SHPRH	ENSG00000146414	1.69	8A	887	wt
SLC35F5	ENSG00000115084	2.2	10T	1157	hmz
SMAP1	ENSG00000112305	1.6	10A	550	hmz
TBC1D23	ENSG00000036054	1.66	9A	1901	wt
TFE3	ENSG00000068323	2.39	8G	1676	wt
TGFBR2*	ENSG00000163513	2.45	10A	831	hmz
TMEM161B	ENSG00000164180	1.57	8T	651	wt
TTC3	ENSG00000182670	2.42	8A, 7A	1867, 2432	htz
WDR19	ENSG00000157796	1.54	8T	788	htz

The threshold for re-expression was considered significant when the fold change was >1.5 (see the Materials and Methods section). Newly described target genes for instability in MSI CRCs are indicated in bold characters. Hmz: homozygously mutated at the coding repeat tract in HCT116. Htz: heterozygously mutated at the coding repeat tract in HCT116. wt: not mutated at the coding repeat tract in HCT116. Target genes that have been already described to be mutated in MSI CRCs are indicated with an ^*.