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. 2008 Jul;124(3):368–379. doi: 10.1111/j.1365-2567.2007.02783.x

Figure 2.

Figure 2

The Complex II binding site is composed of all regions of Site C. (a) Schematic representation of probes derived from the CD40 ligand (CD40L) 3′ untranslated region Site C (E5-1589) that were used to map the Complex II binding site. (b) In vitro synthesized RNA probes spanning the C site, E5-HaeIII (lanes 1–3), E5-1518 (lanes 4–6), E5-HinfI (lanes 7–9), E5-HΔCA (lanes 10–12) and 1518-HaeIII (lanes 13–15) were used in RNA-electrophoretic mobility shift assay without (lanes 2, 5, 8, 11 and 14) or with (lanes 3, 6, 9, 12 and 15) 15 μg Jurkat/D1.1 cytoplasmic extract. Lanes 1, 4, 7, 10 and 13 show intact probes in the absence of RNase and extract.