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CD22 ligand abundance and masking of the CD22 binding site are unaffected by FcγRIIb expression. Splenocytes from FcγRIIb−, CD22− and ST6Gal I-deficient mice were incubated with biotinylated Sambucus nigra agglutinin (SNA) lectin (a) or sialoside probes (b), followed by allophycocyanin-conjugated streptavidin. Staining of B220+ cells was compared to C57BL/6 controls and traces are representative of cells from three mice in each case.
