Figure 4.

Effects of aspirin and NaS on stimulated Erk phosphorylation. (A–C) Neutrophils were incubated in the absence or presence of 10 mM ASA or NaS, stimulated for 2 min with 100 nM FMLP (A) or 20 μM AA (B), or for 10 min with 1 μg/ml PMA (C), lysed, and analyzed for phosphoactivation state by SDS/PAGE and immunoblotting with an antibody specific for the phosphorylated, activated state of Erk 1 and 2. (D–F) The immunoblots in A–C were stripped and reblotted with an anti-Erk1/2 antibody indifferent to phosphorylation state. Data are representative of three experiments.