Table 2.

Effects of salicylates and PD098059 on adherence of neutrophils to ECV304 cell monolayers

Inhibitor	Neutrophils adherent/high-powered field (0.05 mm2)
	No stimulus	FMLP (100 nM)
None	2.3  ±  0.6	9.2  ±  2.7
NaS (0.1 mM)	1.8  ±  0.9	7.7  ±  4.3
NaS (10 mM)	1.6  ±  0.5	3.8  ±  2.6
ASA (0.1 mM)	3.8  ±  0.8	16.6  ±  7.0
ASA (10 mM)	1.2  ±  0.7	3.2  ±  2.4
PD098059 (100 μM)	1.55  ±  0.4	2.3  ±  0.4

Neutrophils were incubated for 10 min at 37°C with the indicated inhibitors, then stimulated 30 min and analyzed for adhesion to ECV304 cell monolayers, expressed as the number of neutrophils adherent/high-powered field (average of 5 fields counted per experiment). Data shown are the mean ± SEM for four experiments, each done in duplicate.