Table 1.

Primers, probes and thermal cycling conditions of qPCR

Gene	Forward primer (5′ → 3′)	Reverse primer (5′ → 3′)	Probe (5′ → 3′)	Annealing-extenstion
MAGEA1 [15]	gCC gAA ggA ACC TgA CC	ACT ggg TTg CCT CTg TCg	TgT gTg CAg gCT gCC ACC TCC T	90 s, 65°C
MAGEA2 [15]	AAg TAg gAC CCg Agg CAC Tg	gAA gAg gAA gAA gCg gTC Tg	CAT TgA Agg AgA AgA TCT gCC TgT ggg TCT TC	1 min, 60°C
MAGEA3 [14]	gTC gTC ggA AAT Tgg CAg TAT	gCA ggT ggC AAA gAT gTA CAA	AAA gCT TCC AgT TCC TT	1 min, 62°C
MAGEA4 [15]	CCA CTA CCA TCA gCT TCA CTT gC	CTT CTC ggA ACA Agg ACT CTg C	Agg CAA CCC AAT gAg ggT TCC AgC	1 min, 63°C
MAGEA6	gTC gTC ggA AAT Tgg CAg T	gCA ggT ggC AAA gAT gTA CAC	TgC AAg gAA TCg gAA gC	1 min, 65°C
MAGEA10	TAC TgC ACC CCT gAg gAg gTC	TgT ggT ggC AAT TCT gTC CTg	AAA Tgg gAg TgA TCC AAg ATC CTT CCC AC	1 min, 64°C
MAGEA12	ggT ggA AgT ggT CCg CAT Cg	gCC CTC CAC TgA TCT TTA gCA A	Agg CAT CTg ATg ggA gg	1 min, 60°C
MAGEC2	ggg AAT CTg ACg gAT Cgg A	ggA ATg gAA CgC CTg gAA C	TgC TCC TgA AgA AgT CgT CAT gCC TCC	1 min, 64°C
GAGE1,2,8a	CTA gAC CAA gAC gCT ACg TAg A	CCC ATC Agg ACC ATC TTC ACA	CCT ATg Cgg CCC gAg CAg TTC Ag	1 min, 62°C
LAGE2/NY-ESO-1 [15]	CgC CTg CTT gAg TTC TAC C	CAC TgC gTg ATC CAC ATC AAC A	TCA gTA TgT TgC Cgg ACA CAg TgA ACT C	1 min, 62°C
ACTB [14]	ATT gCC gAC Agg ATg CAg AA	gTC ATA CTC CTg CTT gCT gA	TCA AgA TCA TTg CTC CTC CTg AgC gC	1 min, 60°C

^aThis assay detects GAGE1, GAGE2 and GAGE8