Figure 3.
Currents from coinjection of αwtαwt and βαwt (A), αwtαwt and βαm (B), or αwtαm and βαwt (C) dimers activated by a range of concentrations of cAMP. The pulse protocol is as in Figs. 1 and 2. (D) Data are normalized dose–response relations at 60 mV for patches with channels from coinjections of αwtαwt and βαm (circles), αwtαwt and βαwt (squares), and αwtαm and βαm (bows). Superimposed are fits of the αwtαwt and βαm and αwtαm and βαm data to Hill equations of the form as in Fig. 1. The fitted values of K1/2 are 9.8 μM and 29 μM, and of n are 1.4 and 1.1 for channels from αwtαwt and βαm, and αwtαm and βαm injections, respectively, and Imax was set at unity.