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Very-low-power immunofluorescence localization of TSC in rat renal cortex. (A) TSC labeling only. (B) Triple-labeling with the anti-TSC rabbit polyclonal antibody (green), a chicken polyclonal antibody to thick ascending limb Na+–K+–2Cl− cotransporter (blue), and a mouse monoclonal antibody to Na+–Ca2+ exchanger, a con necting tubule marker (red). C shows that no labeling was detected when the anti-TSC antibody was preadsorbed with an excess of the immunizing peptide. (Bar, 250 μm.)
