Figure 3.

Effect of dietary NaCl restriction on TSC expression in rat kidney. (A) Immunoblot of whole-kidney homogenates from Sprague–Dawley rats ingesting dietary Na of 2.2 meq/200 g BW per day (control) or 0.2 meq/200 g BW per day (low NaCl). Each lane was loaded with a protein sample from a different rat. The blot was loaded with 10 μg of total protein per lane. (B) Coomassie blue-stained SDS/12% polyacrylamide gel using the same samples as used in the immunoblot in A. This loading gel established that the accompanying immunoblot (loaded identically, Fig. 3A) was uniformly loaded. (C) Control immunoblot using the same samples but probed with a rabbit polyclonal antibody to Na-K-2Cl cotransporter protein (13). The blot was loaded with 7 μg of total protein per lane.