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Northern blot analysis of poly(A)+RNA isolated from mouse upper small intestinal mucosa and kidney cortex. Blots were hybridized with probes derived from a 900-bp 5′ end fragment of type IIb cDNA or from the full length cDNA of the mouse renal type II Na/Pi-cotransporter. Hybridization to the ribosomal protein L28 mRNA was used to confirm equal loadings. In the case of the NaPi-7 probe, five times less poly(A)+RNA of kidney cortex was loaded.
