Figure 4.

Immunodetection of the type IIb Na/P_i-cotransporter. (A) Western blots of isolated mouse small intestinal brush border membranes (35 μg protein per lane). CB, Coomassie blue staining. Incubation with the first antibody was performed in the absence (lane 1) or presence (lane 2) of the antigenic peptide. (B) Immunofluorescence detection of the type IIb cotransporter in the apical membrane of enterocytes. Incubation with the primary antibody was performed in the absence (Upper) or presence (Lower) of antigenic peptide (100 μg/ml).