Table 1.
Protoplast mortality and the incidence of the HII phase (tightly packed cylinders) and associated ultrastructural morphologies (well-ordered striations and loosely ordered swirls) in protoplasts isolated from leaves of nonacclimated RLD and T8 plants
| RLD | T8 | ||
|---|---|---|---|
| −5.0°C | Protoplast mortality (LOR-HII) | 65% | 44% |
| Incidence of HII morphologies | 63% (32/51) | 37% (33/89) | |
| loosely ordered swirls | 33% (17/51) | 26% (23/89) | |
| well-ordered striations | 33% (17/51) | 15% (13/89) | |
| tightly packed cylinders | 22% (11/51) | 7% (6/89) | |
| −5.5°C | Protoplast mortality (LOR-HII) | 83% | 56% |
| Incidence of HII morphologies | 76% (82/108) | 57% (100/176) | |
| loosely ordered swirls | 15% (16/108) | 45% (80/176) | |
| well-ordered striations | 22% (24/108) | 17% (30/176) | |
| tightly packed cylinders | 44% (47/108) | 9% (16/176) | |
| −6.0°C | Protoplast mortality (LOR-HII) | 87% | 74% |
| Incidence of HII morphologies | 91% (19/21) | 74% (84/113) | |
| loosely ordered swirls | 62% (13/21) | 44% (50/113) | |
| well-ordered striations | 67% (14/21) | 42% (48/113) | |
| tightly packed cylinders | 52% (11/21) | 21% (24/113) |
Protoplast mortality (LOR-HII) was calculated from the survival data after a freeze/hypertonic thaw treatment shown in Fig. 1B. The number before the slash represents the number of protoplasts in which the particular morphology was observed; the number after the slash represents the total number of protoplasts observed.