APPENDIX 1.

Amino acid sequence of various peptides corresponding to hepatitis E virus ORF2 (peptides 1-81) and ORF3 (peptides 82–95) used for lymphocyte proliferation assays

Pool number	Peptide number	Amino acid sequence
1	1	H–MRPRPILLLLLMFLPMLPAP–OH
	2	H–LLLMFLPMLPAPPPGQPSGR–OH
	3	H–LPAPPPGQPSGRRRGRRSGG–OH
	4	H–PSGRRRGRRSGGSGGGFWGD–OH
1	5	H–RSGGSGGGFWGDRVDSQPFA–OH
	6	H–FWGDRVDSQPFAIPYIHPTN–OH
	7	H–QPFAIPYIHPTNPFAPDVTA–OH
	8	H–HPTNPFAPDVTAAAGAGPRV–OH
	9	H–DVTAAAGAGPRVRQPARPLG–OH
2	10	H–GPRVRQPARPLGSAWRDQAQ–OH
	11	H–RPLGSAWRDQAQRPAVASRR–OH
	12	H–DQAQRPAVASRRRPTTAGAA–OH
	13	H–ASRRRPTTAGAAPLTAVAPA–OH
	14	H–AGAAPLTAVAPAHDTPPVPD–OH
	15	H–VAPAHDTPPVPDVDSRGAIL–OH
	16	H–PVPDVDSRGAILRRQYNLST–OH
	17	H–GAILRRQYNLSTSPLTSSVA–OH
	18	H–NLSTSPLTSSVATGTNLVLY–OH
3	19	H–SSVATGTNLVLYAAPLSPLL–OH
	20	H–LVLYAAPLSPLLPLQDGTNT–OH
	21	H–SPLLPLQDGTNTHIMATEAS–OH
	22	H–GTNTHIMATEASNYAQYRVA–OH
	23	H–TEASNYAQYRVARATIRYRP–OH
	24	H–YRVARATIRYRPLVPNAVGG–OH
	25	H–RYRPLVPNAVGGYAISISFW–OH
	26	H–AVGGYAISISFWPQTTTTPT–OH
	27	H–ISFWPQTTTTPTSVDMNSIT–OH
4	28	H–TTPTSVDMNSITSTDVRILV–OH
	29	H–NSITSTDVRILVQPGIASEL–OH
	30	H–RILVQPGIASELVIPSERLH–OH
	31	H–ASELVIPSERLHYRNQGWRS–OH
	32	H–ERLHYRNQGWRSVETSGVAE–OH
	33	H–GWRSVETSGVAEEEATSGLV–OH
	34	H–GVAEEEATSGLVMLCIHGSL–OH
	35	H–SGLVMLCIHGSLVNSYTNTP–OH
	36	H–HGSLVNSYTNTPYTGALGLL–OH
5	37	H–TNTPYTGALGLLDFALELEF–OH
	38	H–LGLLDFALELEFRNLTPGNT–OH
	39	H–ELEFRNLTPGNTNTRVSRYS–OH
	40	H–PGNTNTRVSRYSSTARHRLR–OH
	41	H–SRYSSTARHRLRRGADGTAE–OH
	42	H–HRLRRGADGTAELTTTAATR–OH
	43	H–GTAELTTTAATRFMKDLYFT–OH
	44	H–AATRFMKDLYFTSTNGVGEI–OH
	45	H–LYFTSTNGVGEIGRGIALTL–OH
6	46	H–VGEIGRGIALTLFNLADTLL–OH
	47	H–ALTLFNLADTLLGGLPTELI–OH
	48	H–DTLLGGLPTELISSAGGQLF–OH
	49	H–TELISSAGGQLFYSRPVVSA–OH
	50	H–GQLFYSRPVVSANGEPTVKL–OH
	51	H–VVSANGEPTVKLYTSVENAQ–OH
	52	H–TVKLYTSVENAQQDKGIAIP–OH
	53	H–ENAQQDKGIAIPHDIDLGES–OH
	54	H–IAIPHDIDLGESRVVIQDYD–OH
7	55	H–LGESRVVIQDYDNQHEQDRP–OH
	56	H–QDYDNQHEQDRPTPSPAPSR–OH
	57	H–QDRPTPSPAPSRPFSVLRAN–OH
	58	H–APSRPFSVLRANDVLWLSLT–OH
	59	H–LRANDVLWLSLTAAEYDQST–OH
	60	H–LSLTAAEYDQSTYGSSTGPV–OH
	61	H–DQSTYGSSTGPVYVSDSVTL–OH
	62	H–TGPVYVSDSVTLVNVATGAQ–OH
	63	H–SVTLVNVATGAQAVARSLDW–OH
8	64	H–TGAQAVARSLDWTKVTLDGR–OH
	65	H–SLDWTKVTLDGRPLSTIQQY–OH
	66	H–LDGRPLSTIQQYSKTFFVLP–OH
	67	H–IQQYSKTFFVLPLRGKLSFW–OH
	68	H–FVLPLRGKLSFWEAGTTKAG–OH
	69	H–LSFWEAGTTKAGYPYNYNTT–OH
	70	H–TKAGYPYNYNTTASDQLLVE–OH
	71	H–YNTTASDQLLVENAAGHRVA–OH
	72	H–LLVENAAGHRVAISTYTTSL–OH
9	73	H–HRVAISTYTTSLGAGPVSIS–OH
	74	H–TTSLGAGPVSISAVAVLAPH–OH
	75	H–VSISAVAVLAPHSALALLED–OH
	76	H–LAPHSALALLEDTLDYPARA–OH
	77	H–LLEDTLDYPARAHTFDDFCP–OH
	78	H–PARAHTFDDFCPECRPLGLQ–OH
	79	H–DFCPECRPLGLQGCAFQSTV–OH
	80	H–LGLQGCAFQSTVAELQRLKM–OH
	81	H–QSTVAELQRLKMKVGKTREL–OH
10	82	H–MNNMSFAAPMGSRPCALGLF–OH
	83	H–PMGSRPCALGLFCCCSSCFC–OH
	84	H–LGLFCCCSSCFCLCCPRHRP–OH
	85	H–SCFCLCCPRHRPVSRLAAVV–OH
	86	H–RHRPVSRLAAVVGGAAAVPA–OH
	87	H–AAVVGGAAAVPAVVSGVTGL–OH
	88	H–AVPAVVSGVTGLILSPSQSP–OH
11	89	H–VTGLILSPSQSPIFIQPTPS–OH
	90	H–SQSPIFIQPTPSPPMSPLRP–OH
	91	H–PTPSPPMSPLRPGLDLVFAN–OH
	92	H–PLRPGLDLVFANPPDHSAPL–OH
	93	H–VFANPPDHSAPLGVTRPSAP–OH
	94	H–SAPLGVTRPSAPPLPHVVDL–OH
	95	H–RPSAPPLPHVVDLPQLGPRR–OH