Table 3.
Esterification of E2 by liver microsomes from control and clofibrate-fed ACI rats
| Treatment | E2 mg in pellet | E2-oleoyl-ester formed (pmol/mg protein/min) | E2-oleoyl-ester formed (nmol/liver/min) |
|---|---|---|---|
| 6 weeks | |||
| A. AIN-76A | 0 | 12 ± 2 | 1.10 ± 0.17 |
| B. AIN-76A | 1 | 9 ± 2 | 1.08 ± 0.05 |
| C. 0.4% Clofibrate | 1 | 85 ± 3# | 14.32 ± 1.67# |
| D. AIN-76A | 3 | 8 ± 2 | 0.66 ± 0.15 |
| E. 0.4% Clofibrate | 3 | 74 ± 8# | 13.21 ± 1.91# |
| F. 0.4% Clofibrate | 0 | 72 ± 7* | 12.50 ± 0.69* |
| 12 weeks | |||
| A. AIN-76A | 0 | 15 ± 2 | 1.30 ± 0.08 |
| B. AIN-76A | 1 | 14 ± 2 | 1.23 ± 0.12 |
| C. 0.4% Clofibrate | 1 | 55 ± 9# | 10.53 ± 1.30# |
| D. AIN-76A | 3 | 7 ± 2* | 0.61 ± 0.12* |
| E. 0.4% Clofibrate | 3 | 20 ± 3# | 5.22 ± 1.26# |
| F. 0.4% Clofibrate | 0 | 28 ± 5* | 4.65 ± 0.91* |
Liver microsomes (1 mg protein/ml) were incubated at 37 °C for 30 min with 50 μM [3H]-E2 (3 μCi), 100 μM oleoyl-CoA. Data are presented as the mean ± S.E. of five animals per group.
*
Statistically different from the control group receiving only a 20 mg cholesterol pellet,
#
Statistically different from the group receiving same amount of E2, P <0.05). (ANOVA followed by Bonferroni hoc post test)