Table 2.

Characteristic protein adsorption parameters from depletion experiments using surface charged and uncharged sepharose

			Experimental
Protein (MW, pI)	Surface (Acronym)	Ion exchange capacity (Ave., mmol/mL)	${(D_i)}_n^{\mathit{max}}$ (mg/mL) (N, R2)	${(W_{B_i}^o)}_n^{\mathit{max}}$ (mg/mL)	(Si)n (N, R2)	$\left[\frac{{(D_i^{\mathit{max}})}_n}{{(D_i^{\mathit{max}})}_r}\right]$
Lysozyme (14.5kDa, 11)	Sulfopropyl (SP)	− 0.22 ± 0.05	1.69 ± 0.09 (16, 0.99)	1.86 ± 0.10	0.91 ± 0.01 (12, 0.99)	6.26 ± 0.41
	Carboxy methyl (CM)	− 0.11 ± 0.03	1.11 ± 0.13 (13, 0.93)	1.48 ± 0.18	0.75 ± 0.02 (13, 0.98)	4.11 ± 0.50
	Quaternary ammonium (Q)	0.22 ± 0.06	0.74 ± 0.11 (16, 0.93)	2.06 ± 0.33	0.36 ± 0.02 (12, 0.90)	2.74 ± 0.42
	Diethyl aminoethyl (DEAE)	0.14 ± 0.04	0.68 ± 0.03 (14, 0.97)	1.12 ± 0.08	0.61 ± 0.03 (10, 0.96)	2.52 ± 0.15
	Octadecyl Sepharose (Neutral)	0	0.27 ± 0.01 (12, 0.97)	0.86 ± 0.05	0.32 ± 0.02 (8, 0.92)	1
Albumin (66.3kDa, 5.5)	Sulfopropyl (SP)	− 0.22 ± 0.05	2.98 ± 0.49 (30, 0.93)	9.14 ± 1.58	0.33 ± 0.02 (16, 0.82)	2.04 ± 0.34
	Carboxy methyl (CM)	− 0.11 ± 0.03	2.98 ± 0.30 (22, 0.84)	7.74 ± 043	0.39 ± 0.02 (25, 0.83)	2.04 ± 0.21
	Quaternary ammonium (Q)	0.22 ± 0.06	5.02 ± 0.47 (17, 0.94)	8.78 ± 0.87	0.57 ± 0.02 (13, 0.91)	3.44 ± 0.33
	Diethyl aminoethyl (DEAE)	0.14 ± 0.04	2.55 ± 0.14 (30, 0.95)	6.99 ± 0.49	0.37 ± 0.02 (22, 0.88)	1.75 ± 0.11
	Octadecyl Sepharose (Neutral)	0	1.46 ± 0.05 (30, 0.98)	3.40 ± 0.16	0.46 ± 0.02 (15, 0.94)	1
IgG (160kDa, 7)	Sulfopropyl (SP)	− 0.22 ± 0.05	4.53 ± 0.20 (14, 0.98)	13.21 ± 0.70	0.34 ± 0.01 (10, 0.92)	1.60 ± 0.19
	Carboxy methyl (CM)	− 0.11 ± 0.03	4.82 ± 0.64 (13, 0.86)	12.34 ± 0.43	0.39 ± 0.03 (7, 0.87)	1.70 ± 0.29
	Quaternary ammonium (Q)	0.22 ± 0.06	5.88 ± 0.60 (12, 0.94)	13.03 ± 0.43	0.45 ± 0.02 (7, 0.97)	2.07 ± 0.29
	Diethyl aminoethyl (DEAE)	0.14 ± 0.04	3.58 ± 1.10 (12, 0.93)	9.68 ± 2.98	0.37 ± 0.01 (10, 0.92)	1.26 ± 0.42
	Octadecyl Sepharose (Neutral)	0	2.84 ± 0.23 (15, 0.96)	9.50 ± 0.16	0.30 ± 0.01 (10, 0.89)	1