Table 3.
Theoretical Protein Adsorption Parameters from Depletion Experiments
| Proteins and Surfaces | Theory | If (VI)n = (VI)r | ||||
|---|---|---|---|---|---|---|
| Protein (MW, pI) | Surface (Acronym) | (PiVI)n | (Pi)n | |||
| Lysozyme (14.5kDa, 11) | Sulfopropyl (SP) | 303255 ± 3333 | 21.49 ± 3.28 | 21.49 ± 3.28 | 11108 ± 1698 | 9.32 ± 0.15 |
| Carboxy methyl (CM) | 89977 ± 2400 | 6.38 ± 0.90 | 6.38 ± 0.90 | 3296 ± 464 | 8.10 ± 0.14 | |
| Quaternary ammonium (Q) | 16871 ± 938 | 1.20 ± 0.15 | 1.20 ± 0.15 | 618 ± 78 | 6.43 ± 0.13 | |
| Diethyl aminoethyl (DEAE) | 45937 ± 2278 | 3.25 ± 0.51 | 3.25 ± 0.51 | 1683 ± 262 | 7.43 ± 0.16 | |
| Octadecyl Sepharose (Neutral) | 14114 ± 882 | 1 | 1 | 517 | 6.25 | |
| Albumin (66.3kDa, 5.5) | Sulfopropyl (SP) | 14282 ± 757 | 0.57 ± 0.05 | 0.57 ± 0.05 | 93 ± 12 | 4.53 ± 0.13 |
| Carboxy methyl (CM) | 18484 ± 732 | 0.73 ± 0.06 | 0.73 ± 0.06 | 120 ± 15 | 4.79 ± 0.12 | |
| Quaternary ammonium (Q) | 39461 ± 1332 | 1.57 ± 0.14 | 1.57 ± 0.14 | 256 ± 34 | 5.54 ± 0.13 | |
| Diethyl aminoethyl (DEAE) | 16972 ± 756 | 0.67 ± 0.05 | 0.67 ± 0.05 | 110 ± 14 | 4.70 ± 0.13 | |
| Octadecyl Sepharose (Neutral) | 25153 ± 717 | 1 | 1 | 163 ± 16 | 5.09 ± 0.10 | |
| IgG (160kDa, 7) | Sulfopropyl (SP) | 15583 ± 457 | 1.22 ± 0.08 | 1.22 ± 0.08 | 55 ± 8 | 4.01 ± 0.15 |
| Carboxy methyl (CM) | 19123 ± 1477 | 1.49 ± 0.10 | 1.49 ± 0.10 | 68 ± 10 | 4.21 ± 0.15 | |
| Quaternary ammonium (Q) | 24530 ± 820 | 1.92 ± 0.07 | 1.92 ± 0.07 | 87 ± 12 | 4.46 ± 0.14 | |
| Diethyl aminoethyl (DEAE) | 17530 ± 476 | 1.37 ± 0.04 | 1.37 ± 0.04 | 62 ± 8 | 4.13 ± 0.14 | |
| Octadecyl Sepharose (Neutral) | 12792 ± 428 | 1 | 1 | 45 ± 6 | 3.81 ± 0.12 | |