Skip to main content
. Author manuscript; available in PMC: 2008 Nov 1.
Published in final edited form as: Mol Ther. 2008 Mar 11;16(5):845–853. doi: 10.1038/mt.2008.39

Figure 2. Elicitation of distinctive humoral responses in HSV amplicon vector-vaccinated 3xTg-AD mice.

Figure 2

(a) Helper virus-free HSVIEAβCMV2 (black bars), HSVIEAβCMVIL-4 (grey bars), and HSVIE1CMV2 (open bars) were delivered subcutaneously thrice to 3xTg-AD mice beginning at 2 months of age (1×106 transduction units per vaccination). Serum was obtained from each vaccinated mouse according to the schema illustrated in Figure 1 and serum samples were analyzed for levels of antibodies binding specifically to the Aβ1-42 peptide in quadruplicate by ELISA. Levels of Aβ-specific antibodies arising from each vaccination were corrected using serum isolated from control mice, and are expressed as endpoint titers. (b) Isotypes of α-Aβ1-42 antibodies were determined by ELISA using sera obtained at the 9-month time point from vaccinated 3xTg-AD mice. Levels of Aβ-specific antibody isotypes arising from each vaccination were corrected using serum isolated from control mice, and are expressed as “Corrected Absorbance @ 450 nm”. Error bars represent standard deviation. “*” equals P<0.05, “**” equals P<0.01, and “+” equals P<0.001 as determined by two-way ANOVA with Bonferroni post-hoc analysis.