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. 2008 Jan 18;391(5):1661–1669. doi: 10.1007/s00216-007-1775-0

Table 1.

Temperature profile of the APEX reactions for the PR and RT oligonucleotides

  Denaturation 95 °C 30 sec
PR chip Hybridisation 40 °C 10 min
Extension 68 °C 15 min
RT chip Hybridisation 40 °C 10 min
Extension 68 °C 15 min
Hybridisation 40 °C 10 min
Extension 66 °C 15 min
Hybridisation 40 °C 10 min
Extension 64 °C 15 min
Hybridisation 40 °C 10 min
Extension 62 °C 15 min
Hybridisation 40 °C 10 min
Extension 60 °C 15 min
Hybridisation 40 °C 10 min
Extension 58 °C 15 min

Ramping of the in situ cycler occurred at 1 °C / s