Table 1.
Temperature profile of the APEX reactions for the PR and RT oligonucleotides
| Denaturation | 95 °C | 30 sec | |
|---|---|---|---|
| PR chip | Hybridisation | 40 °C | 10 min |
| Extension | 68 °C | 15 min | |
| RT chip | Hybridisation | 40 °C | 10 min |
| Extension | 68 °C | 15 min | |
| Hybridisation | 40 °C | 10 min | |
| Extension | 66 °C | 15 min | |
| Hybridisation | 40 °C | 10 min | |
| Extension | 64 °C | 15 min | |
| Hybridisation | 40 °C | 10 min | |
| Extension | 62 °C | 15 min | |
| Hybridisation | 40 °C | 10 min | |
| Extension | 60 °C | 15 min | |
| Hybridisation | 40 °C | 10 min | |
| Extension | 58 °C | 15 min |
Ramping of the in situ cycler occurred at 1 °C / s