FIGURE 1.

PGC-1α has a high binding affinity for PPARγ LBD in response to rosiglitazone. A, ligand-dependent binding of various coactivator LXXLL motifs to the PPARγ LBD by AlphaScreen assays. Background reading with the PPARγ LBD is <200 photons. DMSO, dimethyl sulfoxide. B, relative binding affinity of various coregulator peptide motifs to the PPARγ LBD in the presence of rosiglitazone is determined by peptide competition. Various unlabeled peptides (500 nm) are used to compete with the binding of the biotin-tagged SRC2-3 LXXLL motif to PPARγ.