FIGURE 2.

Binding affinity of various PGC-1α and SRC LXXLL motifs to the purified PPARγ LBD as determined by IC₅₀ values from peptide competition experiments using AlphaScreen assays. A, dose-response curves of the PPARγ LBD to the ID1 and ID2 motifs of PGC-1α in the presence of rosiglitazone ligand. B, the IC₅₀ values of various PGC-1α and SRC LXXLL motifs to the purified PPARγ LBD in the absence (Apo) and presence of rosiglitazone. The numbering scheme of the LXXLL motifs is shown on the top of the sequences.