TABLE 1.
Primers used in this study
| Name | Descriptiona | Primer sequence |
|---|---|---|
| LR1 | URE2 290 rev into p281-4-3′ truncation | 5′-TTCCGGAATTCTGTGACTCATATCCGAAAATGC-3′ |
| LR2 | URE2 812 rev into p281-4-3′ truncation | 5′-TTCCGGAATTCTCAGCGCTTCTCTACGTTCAGC-3′ |
| LR3 | URE2 309 rev into p281-4-3′ truncation | 5′-TTCCGGAATTCTTGTAATTCTGGAATACTCCACGTG-3′ |
| LR4 | URE2 497 rev into p281-4-3′ truncation | 5′-TTCCGGAATTCTAACTCTTGCATTAGGGTTCAC-3′ |
| LR5 | URE2 fw into p281-4-3′ truncations | 5′-AAAAAACTCGAGAATAACAACGGCAACC-3′ |
| LR7 | URE2 98 fw into p281-4-5′ truncation | 5′-AAAAAACTCGAGGTAATATAAATTTTGAATTTTCAACAGG-3′ |
| LR8 | URE2 205 fw into p281-4-5′ truncation | 5′-AAAAAACTCGAGCAAAATAATGATAACGAGAATAATATCAAGAATACC-3′ |
| LR9 | URE2 246 fw into p281-4-5′ truncation | 5′-AAAAAACTCGAGCGACAACAACAACAGGCATTTTCG-3′ |
| LR10 | URE2 rev into p281-4-5′ truncations | 5′-TTCCGGAATTCTACCACGCAATGCCTTG-3′ |
| LR11 | RT-PCR LACZ fw | 5′-GCTGGCTGGAGTGCGATCTTCC-3′ |
| LR12 | RT-PCR LACZ rev | 5′-CCAGACGAAGCCGCCCTGTA-3′ |
| LR17 | RT-PCR PGK1 fw | 5′-CCTTCTTGAACGACTGTGTCGGTCC-3′ |
| LR18 | RT-PCR PGK1 rev | 5′-CATTGTCCAACCCTTGCCAGCC-3′ |
| LR27 | URE2 140 fw into p281-4-5′ truncation | 5′-AAAAAACTCGAGUAAUAACAAUAGCAGUAGUAAUAACAAUAAUGUUCAAAACA-3′ |
| LR28 | URE2 118 fw into p281-4-5′ truncation | 5′-AAAAAACTCGAGAAAACAAUAACAGCGGCCGCAA-3′ |
| LR29 | URE2 180 fw into p281-4-5′ truncation | 5′-AAAAAACTCGAGCAACAGGUGUAAAUAAUAAUAAUAAUAACAAUAGCAGUAGUAAU-3′ |
| LR31 | URE2 309 rev into pUC19 | 5′-TTAACGGGATCCTTGTAATTCTGGAATACTCCACGTG-3′ |
| LR34 | T7+URE2 98 fw into pUC19 | 5′-GGAATTCCATATGTAATACGACTCACTATAGGGAGGTAATATAAATTTTGAATTTTCAACAGG-3′ |
| LR35 | T7+URE2 205 fw into pUC19 | 5′-GGAATTCCATATGTAATACGACTCACTATAGGGAGCAAAATAATGATAACGAGAATAATATCAAGAATA CC-3′ |
| LR43 | URE2 A238G pBSKII rev mutagenesis | 5′-CTGTTGTTGTTGTCGATGTTGTTCTAAGGCATTCTTGA TATTATTCTCGTTATCATTATTTTGG-3′ |
| LR44 | URE2 A150U pBSKII fw mutagenesis | 5′-TTTTCAACAGGTGTAAATAATAATAATAATAACATTAGCAGTAGTAATAACAA TAATGTTCAAAACAATAACAGC-3′ |
| LR45 | URE2 A203C pBSKII fw mutagenesis | 5′-ATAACAGCGGCCGCAATGGTCGCCAAAATAATGATAACGAGAATAATATCAAGAATAC-3′ |
| LR46 | URE2 A203C pBSKII rev mutagenesis | 5′-GTATTCTTGATATTATTCTCGTTATCATTATTTTGGCGACCATTGCGGCCGCTGTTAT-3′ |
| LR47 | URE2 A238G pBSKII fw mutagenesis | 5′-GCCAAAATAATGATAACGAGAATAATATCAAGAATGCCTTGAGGCAACATCGACAACAACAA-3′ |
| LR79 | URE2 A150U pBSKII rev mutagenesis | 5′-GCTGTTATTGTTTTGAACATTATTGTTATTACTA CTGCTAATGTTATTATTATTATTATTTACACCTGTTGAAAA-3′ |
| LR109 | T7+URE2 205 A239G fw into pUC19 | 5′-GGAATTCCATATGTAATACGACTCACTATAGGGAGC AAAATAATGATAACGAGAATAATATCAAG-3′ |
| LR266 | URE2 fw into p281-4-Firefly | 5′-TCCTAGGATCCTAGGATCCTAGGATCTCCGCGGGCTCGAGAATAACAACGGCAACCAAGTGTCG-3′ |
| LR267 | URE2 309 rev into p281-4-Firefly | 5′-TCGTCACGACGTTGTAAAACGACGGGATCCCCGGGAATTCTTGTAATTCTGGAATACT CCACGTGACTCATATC-3′ |
| LR268 | URE2 497 rev into p281-4-Firefly | 5′-TCGTCACGACGTTGTAAAACGACGGGATCCCCGGGAATTCTAA CTCTTGCATTAGGGTTGACAGACACA-3′ |
| LR269 | URE2 812 rev into p281-4-Firefly | 5′-TCGTCACGACGTTGTAAAACGACGGGATCCCCGGGAA TTCTCAGCGCTTCTCTACGTTCAGCCA-3′ |
| LR278 | URE2 Full length rev into p281-4-Firefly | 5′-TCGTCACGACGTTGTAAAACGACGGGATCCCCGGGAATTCTACCACGCAATGCCTTG-3′ |
| LR271 | Firefly Luciferase rev into p281-4-Firefly | 5′-CTTACGCGAAATACGGGCAGACATGGCCTGCCCGGTTATTAT TACAATTTGGACTTTCCGCCCTTC-3′ |
| LR279 | Firefly Luciferase fw into p281-4-Firefly | 5′-CCCGTCGTTTTACAACGTCGTGACGAAGACGCCAAAAACATAAAGAAAGGC-3′ |
a
fw, forward; rev, reverse.