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. 2008 Jul 4;283(27):18711–18720. doi: 10.1074/jbc.M801655200

FIGURE 2.

FIGURE 2.

Trypsin-induced depletion of plasma membrane PIP2 and formation of plasma membrane DAG and cytoplasmic IP3. A, confocal fluorescence images of the GFP-PH probe for PIP2/IP3 before (a) and after (b) 1 μm trypsin treatment and of the YFP-C1 probe for DAG before (c) and after (d) trypsin treatment. B, time courses of cytoplasmic fluorescence, normalized to the initial fluorescence (F/Fo). C, average cytoplasmic fluorescence ratio (F/Fo), with F being the maximal fluorescence after trypsin treatment (only responsive cells were analyzed: 6/6 cells for GFP-PH and 9/12 cells for YFP-C1). D, fluorescence images of the GFP-PH probe before (a) and after (b) 100 μm PAR-2 AP stimulation and of the YFP-C1 probe before (c) and after (d) PAR-2 AP. E, time course of cytoplasmic ratio. F, average cytoplasmic fluorescence ratio after PAR-2 AP treatment; the majority of tested cells showed reciprocal translocation of GFP-PH (7/8 cells) and YFP-C1 (3/4).