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. 2008 Jul 4;283(27):18627–18635. doi: 10.1074/jbc.M802205200

FIGURE 2.

FIGURE 2.

Inhibition of FXa by Pefabloc. The initial velocity (V) of peptidyl substrate hydrolysis (S-2765; 100 μm for wild-type rFXa or 500 μm for mutant rFXa) catalyzed by free FXa (A) (3 nm wild-type rFXa; 10 nm mutant rFXa) or prothrombinase (B) (60 μm PCPS and 20 or 100 nm FVa for wild-type rFXa and mutant rFXa, respectively) was determined at increasing concentrations of Pefabloc. For visual purposes, the data are normalized to V/Vo × 100, where V is the velocity at a given concentration of Pefabloc and Vo is the starting initial velocity. ▪, PDFXa; ○, rFXa; ▴, rFXaI16L; and ⋄, rFXaV17A. The data are representative of two to three similar experiments.