FIGURE 4.
Activity of compounds against live arenaviruses. A, infectious LASV was pretreated with candidate compounds 8C1 (20 μm), 17G8 (10 μm), and 17C8 (10 μm) or solvent control (Me2SO) and added to permissive VeroE6 cells. After 1 h of infection, the cells were washed and incubated for 16 h. The cells were fixed, and infection was detected by immunofluorescence detection of viral antigens as described under “Experimental Procedures.” B, blocking of infection of VeroE6 cells with LASV, JUNV, and MACV with candidate drugs as in A. The bars represent average numbers of infected cells in four independent visual fields. One representative example of several independent infections is shown. DMSO, dimethyl sulfoxide.