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. Author manuscript; available in PMC: 2008 Jun 27.
Published in final edited form as: J Biol Chem. 2006 May 17;281(30):20689–20697. doi: 10.1074/jbc.M602311200

FIGURE 9. Effects of Src and Cas on Src-mediated anchorage-independent growth and migration.

FIGURE 9

A and B, anchorage-independent growth. 20,000 cells were plated in 12-well tissue culture plates. Cell numbers were obtained using a Coulter counter after 11 days. Open bars, cells without Src; closed bars, cells expressing Src. C and D, cell migration assays. 200,000 cells were plated on cell culture inserts (3-µm pore size) in 6-well plates. Cell migration was measured after 72 h as the percent of cells found in the bottom well/total cell number. E, cell migration assays were carried out using 8-µm pore size membranes, and cell migration was examined after 24 h.