Table 2.

Primers used for PCR amplification and sequencing

Primer name	Cytochrome oxidase subunit 1 – primer sequence	Forward or Reverse	Source
Asmit1 (AT1)	5' TTT TTT GGG CAT CCT GAG GTT TAT 3'	Forward	[26]
Asmit2 (AT2)	5' TAA AGA AAG AAC ATA ATG AAA ATG 3'	Reverse	[26]
CO1 (LC1490)	5' GGT CAA CAA ATC ATA AAG ATA TTG G 3'	Forward	[18]
CO2 (HCO2198)	5' TAA ACT TCA GGG TGA CCA AAA AAT CA 3'	Reverse	[18]
BulCox1 (BC1)	5' TTT TTG GWG TTT GAT GTG G 3'	Forward	NHM – current project
BulCox2 (BC2)	5' TGT GGT CTG GTA GGW ACC GG 3'	Forward	NHM – current project
BulCox3 (BC3)	5' CGT GGA AAW CTT ATA TCW GGW GC 3'	Reverse	NHM – current project
BulCox4 (BC4)	5' GCW CCW GAT ATA AGW TTT CCA CG 3'	Forward	NHM – current project
BulCox5 (BC5)	5' CCT TTA AGA GGN CCT ATT GC 3'	Forward	NHM – current project
BulCox6 (BC6)	5' CAA TAA ACC CTA AAA TYC C 3'	Reverse	NHM – current project
BulCox7 (BC7)	5' GCA ATA GGT CCT CTT AAA GG 3'	Reverse	NHM – current project
BulCox8 (BC8)	5' GTA ATA AAA TTA ATW GCA CCT AAA A 3'	Reverse	NHM – current project
BulCox9 (BC9)	5' CCW CCT TCA TTT ATT TT 3'	Forward	NHM – current project
BulCox10 (BC10)	5' GCT AAA TGT AAA G 3'	Reverse	NHM – current project
BulCox11 (BC11)	5' TTT TGG DRT YTG RTG YGG 3'	Forward	NHM – current project
BulCox12 (BC12)	5' GCG TTG ACT CTT TTC AAC 3'	Forward	NHM – current project
BulCox13 (BC13)	5' CWT TRT AYW TAA TTT TTG G 3'	Forward	NHM – current project
BulCox14 (BC14)	5' GGA AAT CAG TAM AYA AAA CCA GC 3'	Reverse	NHM – current project
BulAsmit3 (BAT3)	5' CAT AAT GAA AAT GAG CAA CTA C 3'	Reverse	NHM – current project
BulAsmit4 (BAT4)	5' CAT AAT GAA AAT GAG C 3'	Reverse	NHM – current project
Primer name	Second internal transcribed spacer of the ribosomal gene complex – primer sequence	Forward or Reverse	Source
ETTS1	5' TGC TTA AGT TCA GCG GGT 3'	Reverse	[27]
ETTS10	5' GCA TAC TGC TTT GAA CAT CG 3'	Forward	[27]