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. 2008 Jun 19;3:14. doi: 10.1186/1749-8104-3-14

Figure 4.

Figure 4

Regulation of Gap43, Sfrp1 and Sox13 expression by Phox2b in the chicken spinal cord. (a-l') Chicken neural tubes electroporated at HH12-13 with pCAGGS::Phox2b-IRES-EGFP (a-b', e-f', i-j') or with pCAGGS::Ngn2 together with pCAGGS::EGFP (c-d', g-h', k-l') were analyzed 24 or 48 h after electroporation using DIG-labeled riboprobes recognizing GFP, Gap43, Sfrp1 or Sox13 mRNA. The asterisk in (i') marks cells in the transfected area where Sox13 was clearly down-regulated by Phox2b; it was rather up-regulated in adjacent cells. The asterisk in (l') marks cells in the transfected area where Sox13 was upregulated by Ngn2. Each experiment was repeated three times on different embryos with identical results.