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Overexpression of Gap43 does not mimic the effects of Phox2b on relocation to the mantel layer and axonal growth. (a-f) Vectors expressing either GFP alone (a, b) or Phox2b and GFP (c, d) or Gap43 and GFP (e, f) were electroporated into the spinal cord of HH12-13 chicken embryos and GFP was visualized by immunohistochemistry 48 hae (a, c, e) or 3 days after electroporation (dae) (b, d, f). Each experiment was repeated three times on different embryos with identical results.
