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. Author manuscript; available in PMC: 2008 Jun 27.
Published in final edited form as: J Biol Chem. 2004 Apr 19;279(27):28345–28357. doi: 10.1074/jbc.M400457200

Fig. 10. Protection of ERK activity by pORF3.

Fig. 10

A, cells were transfected with the indicated plasmids, and the endogenous ERK activity present in cell lysates was determined using the MBP assay. The -fold activity was calculated by densitometric scanning of the bands; the ERK activity present in mock-transfected cells (lane 2) was used as a reference. The lower panels show Western blots for expression of pORF3 and HA-tagged CL100 or Pyst1. Arrows indicate the relevant protein bands. B, cell lysates were Western blotted for cellular ERK with anti-phospho-ERK (pERK) or anti-ERK antibodies.