Table 3.
Susceptibility of Plasmid Transformants (AG100/Kan pJB1–35) to Chloramphenicol and Penicillin G
| MIC2 |
||
|---|---|---|
| Cell Type/Plasmid1 | Cm | Pn G |
| AG100/Kan | 5 ± 0.9 | 21 ± 3 |
| AG112 | 9 ± 0.8 | 61 |
| pJB-1 | 11 ± 1 | - 3 |
| pJB-3 | 9 ± 1 | 30 ± 6 |
| pJB-5 | 6 ± 0.6 | 30 ± 7 |
| pJB-6 | 12 ± 2 | 32 ± 7 |
| pJB-11 | 10 ± 1 | 30 ± 5 |
| pJB-12 | 10 ± 0.8 | - 3 |
| pJB-20 | 10 ± 1 | 36 |
| pJB-21 | 10 ± 0.9 | - 3 |
| pJB-22 | 13 ± 2 | 43 ± 11 |
| pJB-28 | - 3 | 40 |
| pJB-31 | 9 ± 0.8 | 32 |
| pJB-32 | 11 ± 1 | 30 ± 7 |
| pJB-35 | 6 ± 0.8 | 26 ± 4 |
| DW2/pNOEC73 | -3 | ≥ 80 |
| AG100/Kan/pRU600 | ≥ 35 | - 3 |
Numbers in the plasmid designations indicate the particular isolate from which the plasmid originated.
MIC units are in μg/mL. Results were obtained by using the gradient plate method. The overlay was constructed by supplementing L agar with chloramphenicol (Cm) or penicillin G (Pn G) in the absence or presence of 5 mM salycilate. Transformant Bacteria in log phase were inoculated 1–3 hours after addition of the overlay with sterile cotton swabs. The plates were incubated for 40 hours at 30°C. MIC’s were determined for growth between 40–70% along the gradient. Results are average values from five independent experiments with 15 replications.
Not determined