Table 3.

Thermal melting temperatures (T_m) of OPdA-modified oligonucleotides^a

Oligonucleotide	Tm (unmodified)	ΔTm
5′-TCGTT-OPdA-TTGCT-3′ (12) 5′-AGCAA——T——AACGA-5′	46° C (43° C)	3° C
5′-CAGTC-OpdA-CTAGA-3′ (13) 3′-GTCAG T——GATCT-5′	44° C (44° C)	0° C
5′-CAGTG-OPdA-GTAGA-3′ (14) 3′-GTCAC T——CATCT-5′	46° C (45° C)	1° C
5′-GCAAAAA-OPdA-AAAACATGG-3′ (15) 3′-CGTTTTT——T——TTTTGTACC-5′	55° C (56° C)	−1° C
5′-GCAAAAA-OPdA-AAAACATGG-3′ (15) 3′-CGTTTTT—————TTTTGTACC-5′	51° C (50° C)	1° C
5′-GCAAAAA-OPdA-AAAACATGG-3′ (15) 3′-CGTTTTT—————TTTGTACC-5′	48° C (47° C)	1° C

^aConditions: 100 mM NaCl, 10 mM, pH 7 sodium phosphate, 50 μM EDTA, 0.5 A₂₆₀/mL of each oligonucleotide. The temperature was raised 1° C/min.