Table 3.
C. albicans insertion mutant summary
| ORF | Genea | Clone nameb | Genelength (nt) | Insertion sitec (nt) | S. cerevisiaeortholog (best match)d | No. of isolatesscreenede | No. Recoveredf | Mutantstrain name | Caspofungin growth | Description |
|---|---|---|---|---|---|---|---|---|---|---|
| 19.1032 | SKO1 | CAGMJ28 | 1212 | 1014 | SKO1 | 24 | 1 | JMR061 | − | Putative transcription factor |
| 19.1217 | 19.1217 | CAGJI41 | 2637 | 2289 | YMR247C | 12 | 2 | JI41-8 | + | Predicted ORF |
| 19.1219 | 19.1219 | CAGJS77 | 1202 | 836 | (RKR1) | 12 | 0 | n/a | n/a | Predicted ORF |
| 19.1354 | MSN2 | CAGIX50 | 2718 | 2338 | MSN2 | 12 | 0 | n/a | n/a | Transcription factor involved in stress response |
| 19.1464 | 19.1464 | CAGJG54 | 1839 | 375 | None | 12 | 5 | JG54-7 | + | Predicted ORF |
| 19.1476 | 19.1476 | CAGL856 | 1632 | 261 | IME4 | 12 | 1 | JMR090 | + | Predicted ORF |
| 19.1574 | 19.1574 | CAGIW50 | 1767 | 1274 | TAF7 | 12 | 0 | n/a | n/a | Predicted ORF |
| 19.166 | ASG1 | CAGJ631 | 2973 | 1979 | ASG1 | 12 | 0 | n/a | n/a | Predicted zinc finger transcription factor |
| 19.1685 | ZCF7 | CAGN980 | 1350 | 808 | (YPR196W) | 12 | 4 | JMR049 | + | Putative transcription factor |
| 19.1973 | HAP5 | CAGNV37 | 1047 | 815 | (HAP5) | 12 | 8 | JMR053 | + | Putative transcription factor |
| 19.2012 | NOT3 | CAGHL11 | 2397 | 1705 | (NOT3) | 12 | 11 | HL11-1 | + | Putative transcription factor |
| 19.2105 | 19.2105 | CAGK273 | 651 | 159 | CWC2 | 12 | 1 | JMR091 | + | Predicted ORF |
| 19.232 | 19.232 | CAGNJ15 | 915 | 506 | MED7 | 12 | 0 | n/a | n/a | Putative transcription factor |
| 19.2381 | 19.2381 | CAGLR79 | 939 | 156 | None | 24 | 0 | n/a | n/a | Predicted ORF |
| 19.2580 | HST2 | CAGOP19 | 996 | 758 | HST2 | 12 | 0 | n/a | n/a | Predicted ORF |
| 19.2646 | ZCF13 | CAGIS08 | 3729 | 2651 | (HAP1) | 24 | 2 | IS08-7 | −g | Predicted zinc finger transcription factor |
| 19.2654 | RMS1 | CAGLP16 | 1659 | 926 | SET7 | 24 | 0 | n/a | n/a | Predicted ORF |
| 19.2736 | 19.2736 | CAGKZ07 | 441 | 399 | BUR6 | 12 | 3 | JMR074 | + | Predicted ORF |
| 19.2747 | RGT1 | CAGHK21 | 3090 | 2850 | (RGT1) | 12 | 8 | HK21-1 | + | Putative transcription factor |
| 19.2748 | ARG83 | CAGLE67 | 2925 | 484 | (ARG81) | 12 | 2 | JMR085 | + | Predicted zinc finger transcription factor |
| 19.2808 | ZCF16 | CAGJ869 | 3237 | 1778 | (CAT8) | 12 | 2 | J869-9 | + | Predicted zinc finger transcription factor |
| 19.2963 | 19.2963 | CAGJ120 | 921 | 32 | (HST1) | 12 | 2 | J120-1 | + | Predicted ORF |
| 19.3012 | ARO80 | CAGJA54 | 3198 | 2429 | ARO80 | 12 | 2 | JA54-3 | + | Predicted ORF |
| 19.3035 | 19.3035 | CAGHK81 | 4233 | 2931 | CHD1 | 12 | 6 | HK81-2 | + | Predicted ORF |
| 19.3130 | 19.3130 | CAGLQ69 | 996 | 225 | YLR243W | 12 | 0 | n/a | n/a | Predicted ORF |
| 19.3242 | 19.3242 | CAGJX76 | 735 | 450 | TAF10 | 12 | 0 | n/a | n/a | Predicted ORF |
| 19.3833 | 19.3833 | CAGLP47 | 4201 | 2667 | TFC3 | 12 | 0 | n/a | n/a | Putative transcription factor |
| 19.391 | UPC2 | CAGOF87 | 2139 | 874 | UPC2 | 12 | 2 | JMR038 | + | Transcription factor regulating ergosterol synthesis |
| 19.3912 | GLN3 | CAGKJ50 | 2049 | 1237 | GLN3 | 12 | 0 | n/a | n/a | Transcription factor regulating filamentation |
| 19.4194 | 19.4194 | CAGN140 | 1092 | 24 | TFB4 | 12 | 0 | n/a | n/a | Putative transcription factor |
| 19.4420 | 19.4420 | CAGL823 | 2403 | 981 | (RRN6) | 12 | 0 | n/a | n/a | Predicted ORF |
| 19.4524 | ZCF24 | CAGHM72 | 2185 | 776 | (ASG1) | 12 | 8 | HM72-5 | + | Predicted zinc finger transcription factor |
| 19.4545 | SWI4 | CAGJP77 | 3000 | 284 | (SWI4) | 12 | 0 | n/a | n/a | Putative transcription factor |
| 19.4628 | 19.4628 | CAGNF22 | 1497 | 13 | MPE1 | 12 | 0 | n/a | n/a | Predicted ORF |
| 19.4722 | 19.4722 | CAGP462 | 825 | 269 | RTG1 | 12 | 1 | JMR089 | yes | Predicted ORF |
| 19.4775 | CTA8 | CAGQK08 | 2286 | 1429 | (HSF1) | 12 | 2 | JMR044 | + | Putative transcription factor |
| 19.4814 | 19.4814 | CAGQE66 | 336 | 256 | None | 12 | 4 | JMR040 | + | Predicted ORF |
| 19.4851 | TFA1 | CAGQS80 | 1185 | 280 | TFA1 | 12 | 0 | n/a | n/a | Putative transcription factor |
| 19.4853 | HCM1 | CAGP438 | 1740 | 1030 | HCM1 | 12 | 0 | n/a | n/a | Putative transcription factor |
| 19.4882 | 19.4882 | CAGNN71 | 855 | 627 | TFA2 | 20 | 1 | JMR087 | + | Putative transcription factor |
| 19.5097 | CAT8 | CAGM111 | 3171 | 38 | CAT8 | 24 | 0 | n/a | n/a | Putative transcription factor |
| 19.5268 | 19.5268 | CAGJM31 | 513 | 142 | NUT2 | 12 | 0 | n/a | n/a | Predicted ORF |
| 19.5377 | HOS2 | CAGK036 | 1365 | 1068 | HOS2 | 12 | 1 | K036-9 | + | Predicted ORF |
| 19.5501 | YAF9 | CAGLJ16 | 765 | 28 | YAF9 | 12 | 0 | n/a | n/a | Predicted ORF |
| 19.5552 | 19.5552 | CAGL213 | 2208 | 1315 | (CRT10) | 12 | 1 | JMR088 | 0 | Predicted ORF |
| 19.5558 | RBF1 | CAGIR07 | 1605 | 654 | None | 12 | 0 | n/a | n/a | Transcription factor involved in filamentous growth and pathogenesis |
| 19.5666 | 19.5666 | CAGO538 | 441 | 159 | SUB1 | 12 | 3 | JMR035 | + | Predicted ORF |
| 19.5680 | 19.5680 | CAGNU89 | 1176 | 1156 | None | 12 | 0 | n/a | n/a | Predicted ORF |
| 19.5846 | 19.5846 | CAGKB46 | 1485 | 1173 | TFB2 | 12 | 0 | n/a | n/a | Predicted ORF |
| 19.5871 | 19.5871 | CAGJC25 | 2093 | 253 | SNF5 | 12 | 3 | JC25-6 | + | Predicted ORF |
| 19.5910 | 19.5910 | CAGMJ07 | 2280 | 13 | NTO1 | 12 | 6 | JMR062 | + | Predicted ORF |
| 19.5917 | STP3 | CAGJ322 | 1311 | 113 | (STP2) | 12 | 0 | n/a | + | Transcription factor regulating SAP2 & OPT3 |
| 19.5992 | WOR2 | CAGKZ68 | 1341 | 303 | (LYS14) | 12 | 0 | n/a | n/a | Predicted zinc finger transcription factor |
| 19.6109 | TUP1 | CAGKQ96 | 1545 | 349 | TUP1 | 12 | 4 | JMR069 | n/a | Transcriptional corepressor |
| 19.6393 | 19.6393 | CAGLA20 | 1251 | 776 | GTS1 | 12 | 0 | n/a | n/a | Predicted ORF |
| 19.6414 | 19.6414 | CAGHQ12 | 1923 | 205 | None | 12 | 7 | HQ12-3 | + | Predicted ORF |
| 19.6649 | BRF1 | CAGKG39 | 1662 | 1306 | BRF1 | 12 | 0 | n/a | n/a | Putative transcription factor |
| 19.6753 | 19.6753 | CAGJL50 | 450 | 27 | YBR062C | 12 | 0 | n/a | n/a | Predicted ORF |
| 19.6849 | ELC1 | CAGR528 | 303 | 269 | ELC1 | 24 | 3 | JMR046 | + | Predicted ORF |
| 19.7017 | 19.7017 | CAGK324 | 1008 | 413 | YOX1 | 12 | 3 | JMR092 | + | Predicted ORF |
| 19.7046 | MET28 | CAGKQ61 | 522 | 118 | None | 12 | 0 | n/a | n/a | Predicted ORF |
| 19.705 | 19.705 | CAGQ353 | 1350 | 1330 | GCN5 | 12 | 0 | n/a | n/a | Predicted ORF |
| 19.7234 | 19.7234 | CAGKV55 | 1686 | 698 | RSC8 | 8 | 0 | n/a | n/a | Predicted ORF |
| 19.7317 | UGA33 | CAGKZ33 | 1450 | 717 | (UGA3) | 12 | 8 | JMR077 | + | Predicted zinc finger transcription factor |
| 19.7372 | ZCF36 | CAGIU75 | 3327 | 3164 | (HAP1) | 12 | 4 | IU75-1 | + | Predicted zinc finger transcription factor |
| 19.7381 | ZCF37 | CAGJ793 | 1875 | 1144 | (LYS14) | 12 | 5 | J793-2 | + | Predicted zinc finger transcription factor |
| 19.861 | 19.861 | CAGJM52 | 531 | 267 | (YAP6) | 12 | 0 | n/a | n/a | Putative transcription factor |
| 19.9780 | BDF1 | CAGKO55 | 2199 | 2000 | BDF1 | 12 | 0 | n/a | n/a | Putative transcription factor |
a ORF assignments and gene designations are taken from the Candida Genome Database; http://www.candidagenome.org/.
b Clone name refers to the insertion clone used to make each mutant (see Materials and Methods).
c Insertion site is the distance from the ATG initiator of the ORF to the transposon insertion site.
d S. cerevisiae orthologues or closest homologs (in parentheses) are taken from the Candida Genome Database; http://www.candidagenome.org/.
e No. of screened refers to the number of independent transformants from which Arg+Ura+ segregants were derived to screen for homozygotes.
f No. recovered is the number of independent homozygotes identified among the Arg+Ura+ segregants screened.
g Only one isolate with this insertion allele was caspofungin hypersensitive; additional isolates behaved similarly to control strain DAY286.