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. 2008 Jul;19(7):2949–2961. doi: 10.1091/mbc.E07-05-0521

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© 2008 by The American Society for Cell Biology

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Figure 2. — ARH, dyneins, and γ-tubulin complex proteins interact in both membrane and cytosolic fractions. (A) ARH and its interaction partners, dynein HC, dynein IC, GCP2, GPC3, and γ-tubulin, are found in both membrane (P100, lane 3) and cytosolic (S100, lane 2) fractions from L2 cells. Megalin, an integral membrane protein that binds ARH, is detected exclusively in the membrane fraction (lane 3) as expected. L2 cells were homogenized in immunoprecipitation buffer lacking detergent, and the PNS (lane 1) was fractionated into cytosolic (S100, lane 2) and membrane (P100, lane 3) fractions. Equal volumes of the fractions were analyzed by immunoblotting with the indicated antibodies. (B) Dynein HC, dynein IC, GCP2, and γ-tubulin can be co-IPed with ARH IgG from both cytosolic (S100, lane 1) and membrane (P100, lane 4) fractions. None of these proteins were detected after precipitation with preimmune IgG (lanes 2 and 5). Lanes 3 and 6, lysates of cytosolic and membrane fractions (25 μg). Immunoprecipitation was carried out with ARH 3393 or preimmune IgGs on cytosolic (100,000 × g supernatant) and membrane (100,000 × g pellet) fractions and immunoblotted with the indicated antibodies.