. 2008 Jul;19(7):2949–2961. doi: 10.1091/mbc.E07-05-0521

Table 1.

Measurements of centrosome size and staining in wt and Arh^−/− MEFs by epifluorescence microscopy and confocal 3D rendering

	WT MEFs	Arh^−/− MEFs
Epifluorescent microscopic measurements
Number of cells counted	54	61
Total number of centrosomes counted	138	99
Mean no. of centrosomes/cell	2.56±0.23	1.62±0.16
Mean “area” of each centrosome (μm²)	0.77±0.03	0.42±0.02^a
Mean fluorescence intensity of γ-tubulin (per pixel)^a	101.9±3.1	76.53±3.8^a
Mean integrated fluorescence intensity/centrosome^b	8050±459	3516±259
Mean centrosomal area/cell (μm²)^c	2.07	0.78
Confocal 3D rendering measurements
Number of cells counted	20	22
Total number of centrosomes counted	50	44
Mean no. of centrosomes per cell	2.5±0.25	2.0±0.19
Mean volume of each centrosome (μm³)	2±0.17	0.88±0.12
Mean centrosomal volume/cell (μm³)^d	5±0.78	1.8±0.27

Data represented as mean ± SE.

^a Average pixel intensity of γ-tubulin staining at each centrosome (per pixel, scale 0–255).

^b Mean area of centrosome × mean fluorescence intensity of γ-tubulin staining.

^c Mean number of centrosomes per cell × mean area of cach centrosome.

^d Mean number of centrosomes per cell × mean volume of each centrosome.

* In ∼25% of the Arh^−/− MEFs, there was no visible centrosome, and these cells were not included in determining the centrosome area. Hence the reduction in centrosome assembly is more dramatic than is apparent in the “Mean area of each centrosome” and “Mean fluorescence intensity of γ-tubulin at each centrosome.” The “Mean centrosomal area/cell,” which takes into account both the mean number of centrosome/cell, and the area of each centrosome is a more accurate reflection of the effect of ARH knockout on centrosome assembly.