Figure 1.
Rdi1 and filamentous growth. (A) RDI1 deletion does not affect haploid invasive growth. Cells (105) of the indicated strains were spotted on a YPD plate and incubated for 2 d at 30°C. Pictures were taken before and after gentle rinsing with water. Δste20 cells, which fail to invade the agar, served as control. (B) Δrdi1/Δrdi1 cells exhibit reduced diploid pseudohyphal growth. The indicated strains were grown on low nitrogen SLAD medium for 4 d at 30°C. Δste20/Δste20 cells, which are defective in filamentous growth, were used as control. (C) Constitutively active Cdc42 impairs pseudohyphal growth. 3HA-CDC42 and 3HA-CDC42G12V under control of the CDC42 promoter were integrated at the URA3 locus by using an integrative plasmid. The indicated strains were treated as described in B. The results shown in this figure are representative of two independent experiments.