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. 2008 Jul;19(7):2729–2740. doi: 10.1091/mbc.E08-01-0107

Figure 5.

Figure 5.

cIAP1-CR mediates XIAP down-regulation in the presence of dominant negative ubiquitin. (A) HeLa cells were transfected with LacZ, cIAP1-CR, or cIAP1-CR-H588A in the presence of WT-Ub, Ub-K48R, or Ub-4K7R. Cells were collected 24 h after transfection and subjected to Western immunoblot analysis. (B and C) TREx-HeLa cells were transfected with 6myc-XIAP (B) or 6myc-cIAP1, cIAP1-CR (C) and in the presence of WT-Ub, Ub-K48R, Ub-3KR, or Ub-4K7R. Tetracycline was added to induce the expression of cIAP1-CR for the final 4, 8, 18, and 24 h of a total transfection time of 48 h. Cells were collected at the end of the transfection period and subjected to Western immunoblot analysis.