Figure 3.

MCAK is important for Ran aster pole focusing. Ran asters were assembled in IgG-depleted (ΔIgG) or MCAK-depleted (ΔM) extracts in the presence (+MA) or absence of monastrol. MCAK was added back to the MCAK-depleted extract in the presence (ΔM+M+MA) or absence of monastrol (ΔM+M). Bar, 10 μm. (B) Western blot showing the depletion of endogenous MCAK and add-back of GFP-MCAK to endogenous levels (100 nM). (C) Quantification of the percentage of asters with large holes in the center in extracts depleted of MCAK in the absence (ΔM) or presence of monastrol (ΔM+MA). At least 100 structures were scored from three independent experiments.