Figure 1.

HDAC7 associates with PML in HeLa cells. (A) Confocal immunofluorescence microscopy was carried out using anti-HDAC7 antibodies and anti-PML antibodies. PML NBs occur as multiple speckles within the nucleus. (B) HeLa cells were transfected with a PML expression plasmid. Immunostaining was carried out to detect transfected PML and endogenous HDAC7. a–c, anti-PML and anti-HDAC7 antibodies were used to stain transfected and endogenous PML and endogenous HDAC7; d–f, only anti-PML antibodies were applied; g–i, only anti-HDAC7 antibodies were applied. Note that transfected PML was expressed at a much higher level than endogenous PML. All images were taken at the same settings. (C) HeLa whole cell extracts were prepared and immunoprecipitated with beads alone, anti-PML, or anti-HDAC7 antibodies followed by Western blotting with anti-HDAC7 antibodies. (D) An HDAC7 expression plasmid was singly or cotransfected with a PML expression plasmid followed by immunoprecipitation and Western blotting with the indicated antibodies.