Figure 2.

TNF-α treatment results in the sequestration of HDAC7 to PML NBs. (A) HUVECs were treated with or without TNF-α for 20 h followed by immunostaining with anti-HDAC7 and anti-PML antibodies and confocal microscopy. Note that the intensity of PML NBs in the TNF-α treated samples (l) was 5 times greater than that in the untreated cells (b and g). Unless specified, the laser intensity used in the confocal microscopy was the same as that used in b. The laser intensity used in g was fivefold higher than that in b. HUVECs do not express detectable levels of HDAC4 or HDAC5 (data not shown). (B) Confocal microscopic images of TNF-α treated cells immunostained with anti-PML and anti-MEF2 antibodies. Experiments were carried out according to A.