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S2 cells lack a γ-tubulin–containing MTOC during interphase. (A–F) S2 cells stained for MTs (red), γ-tubulin (green), and phosphohistone H3 (blue). Representative cells in early prophase (A), metaphase (B and E), telophase (C), and interphase (D and F). (E and F) γ-tubulin-GFP (green)-expressing stable line. Scale, 5 μm.
