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Quantitation of CD-BLM-mediated cleavage of GT-2. Column A-E corresponds to line-graphs A-E in Figure 9
| Assay Condition | A | B | C | D | E |
|---|---|---|---|---|---|
| 20 μM CD-BLM | 20 μM CD-BLM + 5 μM Co-BLM | 20 μM CD-BLM + 10 μM Co-BLM | 5 μM BLM A5 | 5 μM BLM A5 + 25 μM Co-BLM | |
| ss (T13 + T38) | 438a | 366 | 259 | 668 | 124 |
| ds (T13 + T38) | 72.3 | 46.1 | 27.3 | 213 | 33.7 |
| Average background | 13 | 17 | 15 | 12 | 13 |
| ss to ds ratio | 7.3 ± 1.4 | 12.5 ± 2.5 | 21 ± 14.2 | 3.1 ± 0.3 | 3.6 ± 0.9 |
aArbitrary unit.
