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. 2008 Apr 29;36(11):3522–3530. doi: 10.1093/nar/gkn219

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© 2008 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — First derivative plots (lower) of thermal denaturation profiles (upper) of IDA–IDA snap-to-it probe ‘4’ hybridized with complementary (curve 1) or single-mismatch (curve 3) DNA in the absence of metal ion (EDTA); or with complementary (curve 2) or single-mismatch (curve 4) DNA in the presence of metal ion (NiSO₄). Absorbance was measured at λ = 260 nm. The mismatch selectivity in the absence of metal ion (segment A; ΔT_m = 17°C) increased in the presence of metal ion (segment B; ΔT_m = 21°C) by 4°C.