Table 4.

Mutations in different mutable motifs

	Increase in mutationsa (PW≤Wrandom value)				Pol θ hot spot
Motifs	XP-Vb	Mouse η−/−c	Pol θ in vitro	Mouse η−/−θ−/−d	GCTTTe (5 at –36)	CAATT (6 at 19)	CATCC (11 at 131)
ADK / MHT	4.3 (<0.01)	2.3 (0.02)	0.3 (0.80)	1.0 (0.45)	NO	YES	YES
			2.5 (0.02)
AA / TT	3.7 (<0.01)	2.8 (0.01)	0.5 (0.22)	0.8 (0.71)	YES	YES	NO
			3.3 (<0.01)

^aNumber of mutations in mutable motifs was calculated for the underlined bases (mutable sites). Values listed represent the fold increase in mutations at mutable sites compared with mutations at other sites. Bold italicized numbers represent a significant increase in mutations at mutable motifs (P_{W Wrandom} 0.05), as revealed by using a Monte Carlo procedure (60).

^bMutation spectra from Mayorov et al. (31).

^cCombined spectra from Martomo et al. and Delbos et al. (61,62).

^dMutation spectra from Masuda et al. (16).

^eMutable motifs were compared with the distribution of base substitution error rates made by pol theta, three hotspots on the pol theta error spectrum (Figure 1) significantly correlated with the AA and ADK motifs.