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. 2008 May 8;36(11):3630–3638. doi: 10.1093/nar/gkn244

Figure 2.

Figure 2.

Unwinding of negatively supercoiled pUC19 plasmid DNA by M-[Fe2L3]4+ (A), P-[Fe2L3]4+ (B) and rac-[Fe2L3]4+ (C). The plasmid at the concentration of 2 × 10−4 M (this concentration is related to the monomeric nucleotide content) was mixed with increasing concentrations of the cylinders in 10 mM Tris·HCl (pH 7.4) incubated for 30 min at 25°C and analysed on 1% agarose gel. Lanes 1 and 12, control (non-modified DNA); (A) lanes 2–11, cylinder:base ratio = 0.011, 0.017, 0.022, 0.025, 0.028, 0.030, 0.033, 0.039, 0.044, 0.055, respectively; (B) lanes 2–11, cylinder:base ratio = 0.021, 0.032, 0.037, 0.043, 0.048, 0.054, 0.059, 0.064, 0.075, 0.086, respectively; (C) lanes 2–11, rb = 0.008, 0.015, 0.023, 0.027, 0.030, 0.034, 0.038, 0.046, 0.053, 0.061, respectively. The sc and oc (open circle) indicate supercoiled, and relaxed (nicked) forms of plasmid DNA.