Figure 2.

Targeted cleavage induced by polyamides covalently linked to a bypiridine–copper chelate on a 29-bp intramolecular duplex target. (A) The 5′ radiolabelled duplex was treated, as indicated, by different conjugates at two concentrations (10 nM and 1 µM) in the presence of CuSO₄ and MPA. Cleavage products were separated by electrophoresis in denaturing acrylamide gel. (B) The cleavage patterns observed with the conjugates Bpy5 is schematically described. Vertical bars indicate the sites of cleavage observed on each strand; the lengths of the bars represent the relative abundance of the fragments. The location of the adenines A₂₀ and A₄₂ is indicated near the gel.