Figure 4.

Comparison of IL-8 HPs with different stem-loop designs. The sequences of three HPs are listed in Table 2 as IL-8 HPS1, IL-8 HPS2 and IL-8 HPS3. The hairpin was closed in the blank control and opened after hybridizing with the RNA. The underlined sequences were complementary to the target RNA. Sequences in italics indicate the stem and sequences in bold form the loop. HPS1: 10 bp in the stem and 41 bp in the duplex; HPS2: 8 bp in the stem and 34 bp in the duplex; HPS3: 6 bp in the stem and 27 bp in the duplex. The concentration of IL-8 RNA was 5 nM. The mean and SD of four experiments are shown.