Table 1.

Oligonucleotide sequences for IL-8 and S100A8

Designation	Sequence (5′ to 3′)	5′-label	3′-label
IL-8 HPL0a,b	GAG GGT TGC TCA GCC CTC TTC AAA AAC TTC TCC ACA ACC CTC	Biotin	Fluorescein
IL-8 HPL1a,b	GAG GGT TGC TCA GCC CTC TTC AAA AAC TTC TCC ACA ACC CTC	BiotinTEG	Fluorescein
IL-8 HPL2a,b	TTT TTT TTT GAG GGT TGC TCA GCC CTC TTC AAA AAC TTC TCC ACA ACC CTC	Biotin	Fluorescein
IL-8 HPL3a,b	TTT TTT TTT GAG GGT TGC TCA GCC CTC TTC AAA AAC TTC TCC ACA ACC CTC	BiotinTEG	Fluorescein
IL-8 CPc	TTT TTT TAT GAA TTC TCA GCC CTC	Biotin	–
IL-8 DPc	TTC AAA AAC TTC TCC ACA ACC CTC	–	Fluorescein
IL-8 HPa,b	GAG GGT TGC TCA GCC CTC TTC AAA AAC TTC TCC ACA ACC CTC	Biotin	Fluorescein
S100A8 HPa,b	GTG TCC TCT TTG AAC CAG ACG TCT GCA CCC TTT TTC CTG ATA TAC TGA GGA CAC	Biotin	Fluorescein

^aHairpin probe design was calculated by MFold free web server (27,28).

^bThe target recognition sequences are listed in italic font. The stem sections of the hairpins are underlined.

^cIL-8 CP is the capture probe with biotin label in dual probes detection. IL-8 DP is the detect probe with fluorescein label in dual probes detection.